Bioinformatics

Bioinformatics Analysis

Overview 

Professional bioinformatics support has become an essential part of any NGS based Genomics study. Quick Biology’s bioinformatics service is designed to assist researchers in areas of difficulties, such as Bio-IT, statistics, and data mining. Service is divided into Standard and Advanced service, and customized service plus functional validation of NGS results using our quick validation services are also available.

NGS service quote request, click here!

Unique bioinformatics services from Quick Biology:

1.RNA-seq

Standard Service:

        a.Raw data QC and clean up

        b.Alignment to a reference with mapping statistics

        c.Gene and transcript-based quantitation, TPM/RPKM/FPKM-based quantitation, Raw hit count-based quantitation

        d.Differentially Expressed Gene

        e.Clustering

        f.Gene Ontology

        g.Pathway Analysis

        h.Gene interaction network

Advanced service:

        i.Alternative pre-mRNA splicing

        j.RNA editing

        k.Antisense transcript 

        l.Novel transcripts

        m.Fusion genes/transcript detection 

        Both service will include a final project report with analysis methods, publication-ready graphics, and references

2.Whole Exome Sequencing

        a.Germline variants, compared to reference genome

        b.Somatic mutations if Tumor-Normal pair (SNVs, InDel, CNV)

        c. Pathway Analysis of Variants

        d. Interaction Network Analysis of Variants

3.Whole Genome Sequencing

        a.Germline variants, compared to reference genome

        b.Somatic mutations if Tumor-Normal pair

        c. Pathway Analysis of Variants

        d. Interaction Network Analysis of Variants

4.ChIP Sequencing

        a.Raw data QC and clean up

        b.Alignment to a reference with mapping statistics

        c.Peaking calling with or without control samples

        d.Gene assignment and peak annotation

        e.Visualization

        f.Final project report with analysis methods, publication-ready graphics, and references

5.Combinatorial/Integrative Genomics and customized service

    -Combinatorial

        a.Combine with Cancer Genome Atlas: TCGA)

        b.Combine with data in GEO (Gene Expression Omnibus)

        c.Combine with data in ArrayExpress

        d.Combine with data in Cosmic

        e.Combine with data in GDSC

        f.Combine with data in CCLE

    -Integrative Genomics and customized service

        g.Sequential analysis combining several distinct omics data from same set of samples.

        h.Gene-set analysis to discover novel or using known groups of related genes/molecules.

        i.Pairwise correlation analysis to infer molecular network interactions.

        j.Network analysis using molecular network interactions to identify active or aberrant subgraphs.

        k.Bayesian analysis to guide making correct assumptions to integrate multiple omics data.

        l.Integrative analyses of many tumor types for clinical application.


Turnaround time 

About 1-3 weeks based on specific project.


NGS service quote request, click here!