siRNA Array plate design and layout

siRNA Array - Drug Target I, II and III

Plate Design and Layout:

siRNA Array targeted genes and pathways:

siRNA Array 1: Apoptosis, Cell cycle, Cytoskeleton, DNA damage, HDAC.

	siRNA Target	Gene Title [Common Name]	Pathway
1	BCL2	B-cell CLL/lymphoma 2	Apoptosis
2	CASP3	caspase 3, apoptosis-related cysteine peptidase
3	XIAP	X-linked inhibitor of apoptosis, E3 ubiquitin protein ligase
4	AURKB	aurora kinase B	Cell cycle
5	CDK1	cyclin-dependent kinase 1
6	CDK4	cyclin-dependent kinase 4
7	MDM2	MDM2 proto-oncogene, E3 ubiquitin protein ligase
8	PLK1	polo-like kinase 1
9	FNTA	farnesyltransferase, CAAX box, alpha
10	KIF11	kinesin family member 11
11	RPS6KA3	ribosomal protein S6 kinase, 90kDa, polypeptide 3
12	PTK2	protein tyrosine kinase 2 [FAK]	Cytoskeleton
13	ROCK1	Rho-associated, coiled-coil containing protein kinase 1
14	MAPT	microtubule-associated protein tau
15	ATM	ATM serine/threonine kinase	DNA Damage
16	CHEK1	checkpoint kinase 1
17	PARP1	poly (ADP-ribose) polymerase 1
18	TOP1	topoisomerase (DNA) I
19	TOP2A	topoisomerase (DNA) II alpha
20	PRKDC	protein kinase, DNA-activated, catalytic polypeptide [DNAPK]
21	PPP1R15A	protein phosphatase 1, regulatory subunit 15A [GADD34]
22	Background (without cells)		(Control)
23	Scrambled siRNA
24	Fluorescent siRNA

siRNA Array 2: Kinase signaling pathways (MEK-ERK, MTOR-S6, NF-κB, NRTK, PAK, PI3K-AKT, PRKC),NOTCH and TNFα signaling pathways.

	siRNA Target	Gene Title [Common Name]	Pathway
1	BRAF	B-Raf proto-oncogene, serine/threonine kinase	MEK-ERK
2	MAPK8	mitogen-activated protein kinase 8 [JNK1]
3	MAP2K1	mitogen-activated protein kinase kinase 1 [MEK1]
4	NTRK1	neurotrophic tyrosine kinase, receptor, type 1
5	MAPK1	mitogen-activated protein kinase 1 [p38]
6	PPM1D	protein phosphatase, Mg2+/Mn2+ dependent, 1D
7	PTPN6	protein tyrosine phosphatase, non-receptor type 6 [SHP1]
8	MTOR	mechanistic target of rapamycin (serine/threonine kinase)	MTOR-S6
9	RPS6KB1	ribosomal protein S6 kinase, 70kDa, polypeptide 1 [p70 S6KA]	MTOR-S6
10	IKBKE	inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon [IKKE]	NF-KB
11	NFKB1	nuclear factor of kappa light polypeptide gene enhancer in B-cells 1	NF-KB
12	APH1A	APH1A gamma secretase subunit	NOTCH
13	PSENEN	presenilin enhancer gamma secretase subunit	NOTCH
14	ABL1	breakpoint cluster region/ABL proto-oncogene 1, non-receptor tyrosine kinase	NRTK
15	BMX	BMX non-receptor tyrosine kinase
16	ITK	IL2-inducible T-cell kinase
17	PAK1	p21 protein (Cdc42/Rac)-activated kinase 1	PAK
18	AKT1	v-akt murine thymoma viral oncogene homolog 1	PI3K-AKT
19	PDK1	3-phosphoinositide dependent protein kinase 1
20	PIK3CA	phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha
21	PRKCA	protein kinase C, alpha	PRKC
22	Background (without cells)		(Control)
23	Scrambled siRNA
24	Fluorescent siRNA

siRNA Array 3: Rac GTPases, Retinoic acid X family, RTK, SMO,Protein modifications, Metabolism.

	siRNA Target	Gene Title [Common Name]	Pathway
1	ARFGAP1	ADP-ribosylation factor GTPase activating protein 1	Rac GTPases
2	RAC1	ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)	Rac GTPases
3	RXRA	retinoid X receptor, alpha	Retinioic acid X family
4	ABL1	ABL proto-oncogene 1, non-receptor tyrosine kinase	RTK
5	ALK	anaplastic lymphoma receptor tyrosine kinase
6	AURKA	aurora kinase A
7	BTK	Bruton agammaglobulinemia tyrosine kinase
8	EGFR	epidermal growth factor receptor
9	FGFR1	fibroblast growth factor receptor 1
10	FLT3	fms-related tyrosine kinase 3
11	IGF1R	insulin-like growth factor 1 receptor
12	MET	MET proto-oncogene, receptor tyrosine kinase
13	SRC	SRC proto-oncogene, non-receptor tyrosine kinase
14	SYK	spleen tyrosine kinase
15	KDR	kinase insert domain receptor [VEGFR2]
16	SMO	smoothened, frizzled class receptor	SMO
17	HSPA4	heat shock 70kDa protein 4 [HSP70]	Protein modification
18	HSP90AA1	heat shock protein 90kDa alpha (cytosolic), class A member 1 [HSP90]
19	PSMC5	proteasome 26S subunit, ATPase 5
20	P4HA1	prolyl 4-hydroxylase, alpha polypeptide I
21	PRKAA1	protein kinase, AMP-activated, alpha 1 catalytic subunit [AMPK]	Metabolism
22	Background (without cells)		(Control)
23	Scrambled siRNA
24	Fluorescent siRNA