1. Improved ATAC-seq for Tissue and Cells
Quick Biology has improved the ATAC-seq method for chromatin accessibility profiling that works across multiple applications with substantial improvement of signal-to-background ratio and information content. Our ATAC protocol enables chromatin accessibility profiling from material that was previously deemed intractable including frozen tissue samples (sections and fragments).
2. RNA/DNA Methylation Analysis (WGBS, m6A and 5mc RNA-seq)
RNA/DNA methylations play a significant regulatory role in diverse biological processes. Nowadays, the importance of RNA/DNA modifications and RNA/DNA methylation, in particular, is evident. Several RNA modifications, including N6-methyladenosine (m6A), 5-methylcytidine (5mC), and 5-hydroxylmethylcytidine (5hmC), are found internally in eukaryotic mRNA and can influence the metabolism and function of mRNA.
To help researchers to identify the location of RNA/DNA modifications and to reveal these modifications’ distinct distribution patterns throughout the transcriptome. Quick Biology is now offering two types of RNA/DNA methylation sequencing service for 5mc and m6A:
- WGBS and for 5mC/5hmC and RRBS Methyl-Seq
- 5mC/5hmC RNA Bisulfite-Seq
- m6A RNA-seq (MeRIP-seq, Methylated RNA Immunoprecipitation)
3. Quick Biology 10X Chromium Single Cell Sequencing
Single cell analysis enables researchers to gain novel insights across a diverse set of research areas, including developmental biology, tumor heterogeneity and disease pathogenesis and progression. It permits comparison of the genomics/transcriptomes/epigenome of individual cells and allows assessment of similarities and differences within a population of cells.
Quick Biology’s 10x Genomics' Chromium single cell service is able to deliver a complete solution for:
- Single Cell Transcriptomics (Gene Expression Profiling, and T/B cells Immune Profiling)
- Single Cell Epigenomics (Chromatin Accessibility, single cell ATAC-seq)
4. Quick Biology low-input / single cell WGS
Obtain whole genome sequences with comprehensive genome coverage and exceptional sequence fidelity from single cells or low input gDNA.
Quick Biology’s single cell / low-input WGS service (scWGS) delivers:
- High sensitivity: Typical yield is greater than 5 µg when starting with 1 ng of genomic DNA. Whole genome amplification from as low as 6 pg DNA (5 human cells).
- Reduced amplification bias and no primer artifacts: Primer-free amplification method utilizes a combination of TthPrimPol Primase (to synthesize primers) and Phi29 DNA polymerase.
- Obtain higher genome coverage and greater sequence fidelity.
5. Quick Biology NGS-based HLA and ABO Typing
Quick Biology’s HLA typing takes advantage of the NGS technology and combines long-range PCR amplification of HLA genes (11 loci) with sequencing on the Illumina MiSeq platform. NGS-based HLA-typing provides high-resolution, unambiguous, and phase-resolved results with quick turnaround time.
Quick Biology is now offering two types of NGS-based HLA typing solutions and a NGS-based ABO typing:
- Illumina TruSight® HLA v2 Sequencing Panel
- Omixon Holotype HLA typing assay
- NGS-Based ABO Typing (covering Exons 2-7 of the ABO gene)
6. Liquid Biopsy, Immune Repertoire Profiling and Cancer Panels
Quick Biology is partnering with ArcherDX for targeted sequencing applications that generate enriched sequencing libraries to detect gene fusions, point mutations, copy number variants (CNVs), and RNA abundance.
- Liquid Biopsy: Archer® Reveal ctDNA™ for Illumina for targeted next-generation sequencing (NGS) of circulating cell-free tumor DNA (ccfDNA/cfDNA/ctDNA) from 28 genes commonly found mutated in solid tumor type cancers.
- Immuno Profiling: Archer® Immunoverse™ for targeted NGS assays to characterize the human immune repertoire (T-cells and B-cells) from RNA input.
- Cancer Panels: Archer® FusionPlex®, VariantPlex® and Reveal-ctDNA™ targeted sequencing approach to characterize gene fusions, CNVs and other variants from a single, low-input FFPE sample and liquid biopsy.